Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Foxp3

Cell type

Cell type Class
Blood
Cell type
iTreg
NA
NA

Attributes by original data submitter

Sample

source_name
iTreg cells
strain
C57BL/6
cell type
iTreg cells
antibody
Rabbit Anti-Foxp3( Nature. 2007;445:936-40.

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
~50-100 x 106 cells for each ChIP were crosslinked with formaldehyde, lysed, and fragmented with a Misonix Sonicator 3000. Following immunoprecipitation with antibody or IgG control, the protein-DNA crosslinks were reversed and DNA was purified and used for the preparation of Illumina sequencing libraries Purified ChIP DNA was used to prepare ChIP-seq libraries using the Illumina TruSeq DNA Sample Preparation v2 kit

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
38279228
Reads aligned (%)
94.6
Duplicates removed (%)
27.8
Number of peaks
1284 (qval < 1E-05)

mm9

Number of total reads
38279228
Reads aligned (%)
94.3
Duplicates removed (%)
27.8
Number of peaks
680 (qval < 1E-05)

Base call quality data from DBCLS SRA